Cell Isolation
Sample Processing Considerations on Older Blood
Problem:
Granulocytes will start to de-granulate after 24h.
Ficoll older blood will results in granulocytes contamination in the PBMC layer.
Solution:
Use RosetteSep Granulocytes Depletion Kit (#15624) to make granulocytes ‘heavy’ so they go through the ficoll again.
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Stem Cell细胞分选的研究与应用