Cell Isolation
General Sample Processing Tips for Human /Mouse Samples
Human
Samples :
•Peripheral Blood, Buffy Coat, Mobilized Blood, Bone Marrow, Cord Blood
Anticoagulants:
•Sodium Citrate (ACD), Heparin, EDTA
•ACD is better for older blood (> 24 hrs). EDTA is only good for fresh blood (<24 hrs), as high concentration of EDTA can affect cell viability.
Donor info, frequency of cell types, and storage conditions:
•Store blood at RT (not in fridge) for up to 3 days. Some cells have a lifespan of a max. 24 hrs outside the body (i.e. Dendritic cells, plasma cells, granulocytes).
Mouse
Anticoagulants:
•Not required for Spleen or Bone Marrow
•ACD, EDTA or Heparin for Blood
Donor info, frequency of cell types, and storage conditions:
•Storage of mouse samples longer than 24 hours is not recommended.